Chemical stress on compounds was pointed out as one of the causes of bacterial antibiotic-resistance and observed in S. aureus whether various substances in daily life affect changes in the antibiotic-resistance. It has been confirmed that pesticides and heavy metal ions such as Cr ion affect antibiotic resistance. In addition, even though each of the two substances that does not affect changes in the antibiotic resistance in a condition, changes in the resistance are possible under mixed conditions, and these changes have also been confirmed in other bacteria in the Pseudomonas genus. It is necessary to add antibiotic resistance tests to the safety test of commercial chemicals, and furthermore, it is necessary to add effects on antibiotic resistance mutations using final whole products.