The primary goal of this research is to examine the expression of a few genes related to bean growth under two distinct experimental conditions: distilled water and PAW irrigation. The amount of chlorophyll, carotenoids, also the expression of some genes involved in the germination process, the rate, speed of germination and the phenotypic analysis of the samples were determined . In order to find the optimal water activation time we did different analyses to find out the water characteristics. The results of our study are positive regarding the efficiency of the method. The growth of plants, watered with PAW for 1 and 5 minutes, was more accelerated compared to the different activation situations.